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Rabbit anti-Leo1 Antibody
Catalog Number: orb1530586
| Catalog Number | orb1530586 |
|---|---|
| Category | Antibodies |
| Description | Leo1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 550 and 600 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | Leo1 |
| UniProt ID | Q8WVC0 |
| NCBI | NP_620147.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | RDL; replicative senescence down-regulated leo1-li Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of mouse Leo1 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Leo1 antibody orb1530586 at 0.04 µg/ml.
Detection of human Leo1 by western blot.Samples: Whole cell lysate from HeLa, MCF-7, and Jurkat cells. Antibody: Affinity purified rabbit anti-Leo1 antibody orb1530586 at 0.04 µg/ml.
Detection of human Leo1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Leo1 used at 1:5, 000 (0.2µg/ml).
Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 used at 1:5, 000 (0.2µg/ml).
Detection of human Leo1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-Leo1 antibody.
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