Rabbit LASP1 Antibody

SKU: orb1525108

Description

LASP1 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 175 and 225
• Target: LASP1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lasp-1; LIM and SH3 domain protein 1; metastatic lymph node gene 50 protein; MLN 50; MLN50

Detection of human LASP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-LASP1 antibody orb1525108 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. LASP1 was also immunoprecipitated by rabbit anti-LASP1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human LASP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-LASP1 Cat. No. orb1525110 used at a dilution of 1: 1, 000 (1µg/ml) (left) and Cat. No. orb1525108 used at a dilution of 1: 200 (1µg/ml) (right). Detection: DAB. Counterstain: Hematoxylin (blue).

Quick Database Links

Gene Symbol

LASP1

UniProt

Q14847

RefSeq (Protein):NP_006139.1