Rabbit anti-LAG3 Recombinant Monoclonal Antibody

• Catalog Number: orb1806463
• Category: Antibodies
• Description: Rabbit anti-LAG3 Recombinant Monoclonal Antibody
• Target: LAG3
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Immunogen: Between 250 and 300
• UniProt ID: Q61790
• Tested applications: ICC, IHC, WB
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR261L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: lymphocyte activation gene 3 protein; Ly66; LAG-3;
• Note: For research use only

Detection of mouse LAG3 by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3, CTLL-2, NIH 3T3, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806463) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse LAG3 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806463). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse LAG3 by immunocytochemistry. Sample: FFPE section of J774A.1 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806463). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse LAG3 by immunocytochemistry. Sample: FFPE section of CTLL-2 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806463). Secondary: HRP-conjugated goat anti-rabbit IgG.