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Description
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1450 and 1500 |
| Target | KIAA1549 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human KIAA1549 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIAA1549 antibody (orb1520265) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human KIAA1549 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIAA1549 antibody (orb1520265) used for IP at 1 µg per reaction. For blotting immunoprecipitated KIAA1549, orb1520265 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_065961.2 |
|---|
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Protocol Information
Rabbit KIAA1549 Antibody (orb1520265)
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