Rabbit KAP-1 Antibody

SKU: orb1523536

Description

KAP-1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 175 and 225
• Target: KAP-1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AA408787; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB-A-interacting protein; KRIP-1; MommeD9; RING-type E3 ubiquitin transferase TIF1-beta; Tif1b; Tif1beta; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; tripartite motif protein 28; tripartite motif-containing protein 28

Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells, NIH 3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody orb1523536 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody orb1523536 used for IP at 6 µg/mg lysate.

Quick Database Links

Gene Symbol

KAP-1

UniProt

Q62318

RefSeq (Protein):NP_035718.2