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Rabbit anti-JARID1D/SMCY Antibody
Catalog Number: orb1527856
| Catalog Number | orb1527856 |
|---|---|
| Category | Antibodies |
| Description | JARID1D/SMCY Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 1489 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | JARID1D/SMCY |
| UniProt ID | Q9BY66 |
| NCBI | NP_004644.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | HY; lysine (K)-specific demethylase 5D; Smcy homol Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human SMCY/JARID1D by western blot. Samples: Whole cell lysate (15 µg) from Jurkat, HDLM-2, A-549, 786-O, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMCY/JARID1D antibody orb1527856 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SMCY/JARID1D by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMCY/JARID1D antibody orb1527856 used for IP at 3 µg per reaction.
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