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Catalog Number | orb1527109 |
---|---|
Category | Antibodies |
Description | IP3R3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 650 and 700 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-IP3R3 antibody A302-160A.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | IP3R3 |
UniProt ID | Q14573 |
NCBI | NP_002215.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | insP3R3; type 3 InsP3 receptor; Type 3 inositol 1, Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human IP3R3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IP3R3 antibody orb1527109 used for IP at 3 µg per reaction.
Detection of human IP3R3 by immunohistochemistry.Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-IP3R3 (Cat. No. orb1527109) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB