Rabbit Importin 7 Antibody

SKU: orb1526106

Description

Importin 7 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 225 and 275
• Target: Importin 7
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Imp7; importin-7; RAN-binding protein 7; RANBP7

Detection of human and mouse Importin 7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Importin 7 antibody orb1526106 used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 10 µg/mg lysate. Importin 7 was also immunoprecipitated by rabbit anti-Importin 7 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Detection of human Importin 7 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Importin 7 (Cat. No. orb1526106) used at a dilution of 1: 1, 000 (0.2µg/ml) Detection: DAB

Quick Database Links

Gene Symbol

Importin 7

UniProt

O95373

RefSeq (Protein):NP_006382.1