Rabbit anti-HMCES Antibody

• Catalog Number: orb1520284
• Category: Antibodies
• Description: HMCES Antibody
• Target: HMCES
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 304 and C-terminus
• UniProt ID: Q96FZ2
• Tested applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 20-40 µl/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: 5-hydroxymethylcytosine (hmC) binding, ES cell-spe
• Note: For research use only
• NCBI: Q96FZ2.1

Detection of human HMCES by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-HMCES antibody (orb1520284) used for WB at 0.1 mg/ml. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human HMCES by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-HMCES antibody (orb1520284) used for IP at 2 µg per reaction.