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Catalog Number | orb1519997 |
---|---|
Category | Antibodies |
Description | HIF2-alpha Antibody |
Target | HIF2-alpha |
Clonality | Recombinant |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Whole IgG |
Concentration | 400 µg/ml |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Immunogen | Between 400 and 450 |
UniProt ID | Q99814 |
Tested applications | ChIP, ICC, IHC, IP, WB |
Dilution range | WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections. Overnight incubations are suggested.; ICC - 1:200 - 1:1,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.; ICC-IF - 1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
Application notes | Format: Whole IgG |
Antibody Type | Primary Antibody |
Clone Number | BL-95-1A2 |
Storage | 2 - 8°C |
Alternative names | ECYT4; HIF2-alpha; PAS domain-containing protein 2 Read more... |
Note | For research use only |
NCBI | NP_001421.2 |
Expiration Date | 12 months from date of receipt. |
ChIP, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ChIP, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |