Rabbit anti-HEXIM1 Antibody

• Catalog Number: orb1525439
• Category: Antibodies
• Description: HEXIM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 309 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HEXIM1
• UniProt ID: O94992
• NCBI: NP_006451.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: cardiac lineage protein 1; hexamthylene bis-acetam
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human HEXIM1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEXIM1 antibody orb1525439 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human HEXIM1 by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-HEXIM1 used at a dilution of 1: 200 (1µg/ml) or 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).

Detection of mouse HEXIM1 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. orb1525439) used at a dilution of 1: 200 (1µg/ml). Detection: DAB