Rabbit anti-GCF Antibody

SKU: orb1527554

Description

GCF Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 175 and 225
• Target: GCF
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

TCF-9; Transcription factor 9; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; GC bindng factor; GC binding factor; DNABF; C2orf3; GCF; transcription factor 9 (binds GC-rich sequences)

Detection of human and mouse GCF by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GCF antibody orb1527554 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. GCF was also immunoprecipitated by rabbit anti-GCF antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

GCF

UniProt

P16383

RefSeq (Protein):NP_003194.3