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| Catalog Number | orb431975 |
|---|---|
| Category | Antibodies |
| Description | Rabbit anti Rat GAP43 (pSer41) antibody recognizes growth-associated protein 43 (GAP43), also known as neuromodulin, axonal membrane protein GAP-43, neural phosphoprotein B-50 and pp46, when phosphorylated at serine 41. GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C. |
| Target | Antisera to phosphorylated GAP-43 were raised by repeated immunisations of rabbits with highly purified antigen |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Rat |
| Form/Appearance | Purified IgG - Liquid |
| Buffer/Preservatives | 10mM HEPES pH7.5, 0.09% Sodium Azide, 0.01% rAlbumin, 50% Glycerol |
| Purity | Purified IgG prepared by affinity chromatography |
| Immunogen | Synthetic phosphopeptide corresponding to an amino acid sequence within GAP-43 which includes phosphorylated Ser41 |
| UniProt ID | P17677 |
| MW | 50kDa |
| Tested applications | WB |
| Dilution range | WB: 1/1000 |
| Application notes | Detects a band of approximately 50kDa in rat cortex lysates |
| Cross Reactivity | Monkey,Human,Dog,Chicken,Bovine,Mouse,Zebrafish,Xenopus |
| Storage | This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C |
| Research Area | Developmental Biology, Epigenetics |
| Note | For research use only |

Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at Ser41 using Rabbit anti Rat GAP43 (pSer41) antibody
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