Rabbit anti-EYA4 Antibody

• Catalog Number: orb1526290
• Category: Antibodies
• Description: EYA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EYA4
• UniProt ID: O95677
• NCBI: NP_004091.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: eyes absent-like protein 4; CMD1J; eyes absent hom
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EYA4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EYA4 antibody (orb1526290) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human EYA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EYA4 antibody (orb1526290) used for IP at 6 µg per reaction. EYA4 was also immunoprecipitated by a previous lot of this antibody (orb1526290) and a second antibody against a different epitope of EYA4.