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Catalog Number | orb1523858 |
---|---|
Category | Antibodies |
Description | EPRS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 950 and 1000 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | EPRS |
UniProt ID | P07814 |
NCBI | NP_004437.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | PIG32; Glutamyl-tRNA synthetase; QPRS; QARS; proly Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse EPRS by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-EPRS antibody orb1523858 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human EPRS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EPRS antibody orb1523858 used for IP at 6 µg/mg lysate.
Detection of human EPRS by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-EPRS used at a dilution of 1: 1, 000 (1µg/ml) Detection: DAB