Rabbit anti-EML4 Antibody

• Catalog Number: orb1527571
• Category: Antibodies
• Description: EML4 Antibody
• Target: EML4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 931 and C-terminus
• UniProt ID: Q9HC35
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: ROPP120; echinoderm microtubule-associated protein
• Note: For research use only
• NCBI: NP_061936.2

Detection of human EML4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EML4 antibody orb1527571 used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. EML4 was also immunoprecipitated by rabbit anti-EML4 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human EML4 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-EML4 (Cat. No. orb1527571) used at a dilution of1: 1, 000 (1µg/ml). Detection: DAB