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Catalog Number | orb1527847 |
---|---|
Category | Antibodies |
Description | eIF3D/EIF3S7 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 150 and 200 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | eIF3D/EIF3S7 |
UniProt ID | O15371 |
NCBI | NP_003744.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | EIF3S7; translation initiation factor eIF3 p66 sub Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human eIF3D/EIF3S7 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF3D/EIF3S7 antibody orb1527847 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 3 minutes.
Detection of human eIF3D/EIF3S7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF3D/EIF3S7 antibody orb1527847 used for IP at 3 µg/mg lysate.