Rabbit anti-EBP1 Antibody

• Catalog Number: orb1525485
• Category: Antibodies
• Description: EBP1 Antibody
• Target: EBP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 344 and C-terminus
• UniProt ID: Q9UQ80
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: ErbB-3 binding protein 1; p38-2G4; proliferation-a
• Note: For research use only
• NCBI: NP_006182.2

Detection of human and mouse EBP1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T, 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EBP1 antibody orb1525485 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Detection of human EBP1 by immunohistochemistry. Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1 used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).