Rabbit anti-E-Cadherin Recombinant Monoclonal Antibody

• Catalog Number: orb1519777
• Category: Antibodies
• Description: E-Cadherin Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR088G
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Residues 155-707 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 250 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: E-Cadherin
• UniProt ID: P12830
• NCBI: NP_004351.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: LCAM; CDHE; UVO; epithelial cadherin; epididymis s
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human E-Cadherin in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (orb1519777). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human E-Cadherin (shaded) in HT29 cells by flow cytometry. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody (orb1519777) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.