You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
Rabbit anti-DOT1L Antibody
Catalog Number: orb1529333
| Catalog Number | orb1529333 |
|---|---|
| Category | Antibodies |
| Description | DOT1L Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, IHC, IP, WB |
| Reactivity | Human |
| Immunogen | Between 1000 and 1050 |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | DOT1L |
| UniProt ID | Q8TEK3 |
| NCBI | NP_115871.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | H3-K79-HMTase; histone H3-K79 methyltransferase; l Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1µg/ml).
Detection of human DOT1L by WB and IP.Samples: HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody orb1529333 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).
Localization of DOT1L Binding Sites by ChIP-sequencing. Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing.
- Item 1 of 3
- Item 1 of 1
- Item 1 of 1
- Item 1 of 1
