Rabbit anti-DGKzeta Antibody

• Catalog Number: orb1525685
• Category: Antibodies
• Description: DGKzeta Antibody
• Target: DGKzeta
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 878 and C-terminus
• UniProt ID: Q13574
• Tested applications: IHC, WB
• Dilution range: WB - 1:10,000 - 1:25,000; IP - Not recommended; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: DAGK5; hDGKzeta; diglyceride kinase zeta; diacylgl
• Note: For research use only
• NCBI: NP_001099010.1

Detection of human DGKzeta by western blot. Samples: Whole cell lysate (2 to 5 µg) from HEK293T, HeLa, Hep-G2, Jurkat, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DGKzeta antibody orb1525685 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human DGKzeta by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DGKzeta (orb1525685) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human DGKzeta by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DGKzeta (orb1525685) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB