Rabbit anti-Derlin-1 Antibody

• Catalog Number: orb1525885
• Category: Antibodies
• Description: Derlin-1 Antibody
• Target: Derlin-1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 201 and C-terminus
• UniProt ID: Q9BUN8
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: Der1-like protein 1; derlin-1; Der1-like domain fa
• Note: For research use only
• NCBI: NP_077271.1

Detection of human and mouse Derlin-1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Derlin-1 antibody orb1525885 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Derlin-1 was also immunoprecipitated by rabbit anti-Derlin-1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Detection of human Derlin-1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Derlin-1 (Cat. No. orb1525885) used at a dilution of 1: 200 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate