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| Catalog Number | orb1525666 |
|---|---|
| Category | Antibodies |
| Description | DDX47 Antibody |
| Target | DDX47 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Whole IgG |
| Concentration | 200 µg/ml |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Immunogen | Between 405 and C-terminus |
| UniProt ID | Q9H0S4 |
| Tested applications | IP, WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | DEAD box protein 47; RRP3; probable ATP-dependent Read more... |
| Note | For research use only |
| NCBI | NP_057439.2 |

Detection of human and mouse DDX47 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX47 antibody orb1525666 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DDX47 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX47 antibody orb1525666 used for IP at 6 µg per reaction.
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