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Catalog Number | orb1527981 |
---|---|
Category | Antibodies |
Description | Cyclin L2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 470 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Cyclin L2 |
UniProt ID | Q96S94 |
NCBI | NP_112199.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | HLA-ISO; SB138; paneth cell-enhanced expression pr Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Cyclin L2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, K-562, HeLa, U2OS, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Cyclin L2 antibody orb1527981 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Cyclin L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Cyclin L2 antibody orb1527981 used for IP at 6 µg per reaction.