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Catalog Number | orb1528755 |
---|---|
Category | Antibodies |
Description | CSTF50 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 75 and 125 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CSTF50 |
UniProt ID | Q05048 |
NCBI | NP_001315.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | cleavage stimulation factor subunit 1; CstF-50; CS Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse CSTF50 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-CSTF50 antibody orb1528755 at 0.4 µg/ml.
Detection of human CSTF50 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of mouse CSTF50 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human CSTF50 by western blot of immunoprecipitates.Samples: Whole cell lysate from Hela cells. Antibodies: Affinity purified rabbit anti-CSTF50 antibody.