Rabbit anti-CNOT3 Antibody

• Catalog Number: orb1527115
• Category: Antibodies
• Description: CNOT3 Antibody
• Target: CNOT3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 525 and 575
• UniProt ID: O75175
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: CCR4-NOT transcription complex subunit 3; NOT3; NO
• Note: For research use only
• NCBI: NP_055331.1

Detection of human CNOT3 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-CNOT3 antibody orb1527115 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human CNOT3 by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-CNOT3 (Cat. No. orb1527115) used at a dilution of 1: 200 (1µg/ml). Detection: DAB