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Rabbit anti-CNOT3 Antibody
Catalog Number: orb1527115
| Catalog Number | orb1527115 |
|---|---|
| Category | Antibodies |
| Description | CNOT3 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human |
| Immunogen | Between 525 and 575 |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | CNOT3 |
| UniProt ID | O75175 |
| NCBI | NP_055331.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | CCR4-NOT transcription complex subunit 3; NOT3; NO Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human CNOT3 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-CNOT3 antibody orb1527115 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human CNOT3 by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-CNOT3 (Cat. No. orb1527115) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
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