Rabbit anti-CD70 Recombinant Monoclonal Antibody

• Catalog Number: orb1784577
• Category: Antibodies
• Description: Rabbit anti-CD70 Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR266L
• Tested applications: ICC, IHC, WB
• Reactivity: Human
• Immunogen: Residues 50-193 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 500 µg/ml
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD70
• UniProt ID: P32970
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: surface antigen CD70; tumor necrosis factor ligand
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD70 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, 786-O (10 µg) , HEK293T, THP-1, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784577) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD70 by immunohistochemistry. Sample: FFPE section of human lymph node with metastatic lung cancer. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784577). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD70 by immunocytochemistry. Sample: FFPE section of 786-O cells. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784577). Secondary: HRP-conjugated goat anti-rabbit IgG.