Rabbit CD45 Antibody

SKU: orb1523108

Description

CD45 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:1,000 - 1:5,000. Acetone or Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1256 and C-terminus
• Target: CD45
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B220; CD antigen CD45; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody orb1523108 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody orb1523108 used for IP at 6 µg per reaction.

Detection of human CD45 by immunohistochemistry.Sample: FFPE section of human lung cancer infiltrate. Antibody: Affinity purified rabbit anti-CD45 (Cat. No. orb1523108) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human CD45 by immunocytochemistry. Samples: A: Acetone-fixed Jurkat cells, B: Formaldehyde-fixed Jurkat cells, C: Acetone-fixed Ramos cells, and D: Formaldehyde-fixed Ramos cells. Antibody: Affinity purified rabbit anti-CD45 (Cat. No.orb1523108) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

CD45

UniProt

P08575

RefSeq (Protein):NP_563578.2