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Catalog Number | orb1806448 |
---|---|
Category | Antibodies |
Description | Rabbit anti-CD39 Recombinant Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BLR285L |
Tested applications | ICC, IHC, WB |
Reactivity | Mouse |
Immunogen | Between 300 and 350 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CD39 |
UniProt ID | P55772 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
Alternative names | E130009M23Rik; NTPDase 1; lymphoid cell activation Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse CD39 by western blot. Samples: Whole cell lysate (25 µg) from CTLL-2, CH27, NIH 3T3, J774A1, and WEHI-231 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD39 recombinant monoclonal antibody (orb1806448) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse CD39 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-CD39 recombinant monoclonal antibody (orb1806448). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse CD39 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD39 recombinant monoclonal antibody (orb1806448). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse CD39 by immunocytochemistry. Sample: FFPE section of CTLL-2 cells. Antibody: Rabbit anti-CD39 recombinant monoclonal antibody (orb1806448). Secondary: HRP-conjugated goat anti-rabbit IgG.
ICC, IHC, WB | |
Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |