Rabbit anti-CBX2 Antibody

• Catalog Number: orb1526469
• Category: Antibodies
• Description: CBX2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IP
• Reactivity: Human
• Immunogen: Between 482 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A302-524A has not performed satisfactorily when used for WB of CBX2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CBX2.; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CBX2
• UniProt ID: Q14781
• NCBI: NP_005180.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: modifier 3; Pc class homolog; M33; chromobox prote
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CBX2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBX2 antibody orb1526469 used for IP at 6 µg per reaction.

Localization of CBX2 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX2 antibody orb1526469 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX2 binding within a 1.5 Mb region of chromosome 1 as detected using anti-CBX2 antibody orb1526469.