Rabbit CASC3 Antibody

SKU: orb1526560

Description

CASC3 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 653 and C-terminus
• Target: CASC3
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

barents; ZBT; Zcancer susceptibility 3; cancer susceptibility candidate 3; cancer susceptibility candidate gene 3 protein; metastatic lymph node 51; metastatic lymph node gene 51 protein; MLN 51; MLN51; protein barents; Zprotein CASC3

Detection of human CASC3 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CASC3 antibody orb1526560 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CASC3 was also immunoprecipitated by rabbit anti-CASC3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human CASC3 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CASC3 (Cat. No. orb1526560) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

CASC3

UniProt

O15234

RefSeq (Protein):NP_031385.2