Rabbit anti-BRD3 Antibody

• Catalog Number: orb1526750
• Category: Antibodies
• Description: BRD3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 676 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 5 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRD3
• UniProt ID: Q15059
• NCBI: NP_031397.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: RING3-like protein; bromodomain-containing protein
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BRD3 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD3 antibody orb1526750 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human BRD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD3 antibody orb1526750 used for IP at 6 µg per reaction.

Detection of human BRD3 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma.Antibody: Affinity purified rabbit anti-BRD3 (orb1526750) used at a dilution of 1: 200 (1µg/ml). Detection: DAB