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Rabbit anti-BRD1 Antibody
Catalog Number: orb1526753
| Catalog Number | orb1526753 |
|---|---|
| Category | Antibodies |
| Description | BRD1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 775 and 825 |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | BRD1 |
| UniProt ID | O95696 |
| NCBI | NP_055392.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | BR140-like protein; BRL; bromodomain and PHD finge Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human BRD1 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, HeLa nuclear extract (NE) , Jurkat, HEK293T, and HEK293T nuclear extract (NE) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD1 antibody (orb1526753) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD1 antibody (orb1526753) used for IP at 6 µg per reaction. BRD1 was also immunoprecipitated by a previous lot of this antibody (orb1526753) and a second antibody against a different epitope of BRD1 (Antibody 2).
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