Rabbit anti-BAP1 Antibody

• Catalog Number: orb1526975
• Category: Antibodies
• Description: BAP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: Between 500 and 550
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-BAP1 antibody A302-243A.; IP - 5 - 10 µg/mg lysate; IHC - 1:200 to 1:1,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BAP1
• UniProt ID: Q92560
• NCBI: NP_004647.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BRCA1 associated protein-1 (ubiquitin carboxy-term
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAP1 antibody orb1526975 used for IP at 6 µg per reaction.

Detection of human BAP1 in FFPE prostate carcinoma by IHC.Antibody: Rabbit anti-BAP1 antibody (orb1526975). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.