Rabbit anti-BAP1 Antibody

• Catalog Number: orb1526974
• Category: Antibodies
• Description: BAP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 679 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 5 - 10 µg/mg lysate; IHC - 1:200 to 1:1,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BAP1
• UniProt ID: Q92560
• NCBI: NP_004647.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BRCA1 associated protein-1 (ubiquitin carboxy-term
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BAP1 by western blot. Samples: Whole cell lysate (50 µg) from RKO, HeLa, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAP1 antibody (orb1526974) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human BAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAP1 antibody (orb1526974) used for IP at 6 µg per reaction. BAP1 was also immunoprecipitated by a previous lot of this antibody (orb1526974) and a second antibody against a different epitope of BAP1.

Detection of human BAP1 in FFPE prostate carcinoma by IHC.Antibody: Rabbit anti-BAP1 antibody (orb1526974). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.