Rabbit anti-B7-H6 Recombinant Monoclonal Antibody

• Catalog Number: orb1784568
• Category: Antibodies
• Description: Rabbit anti-B7-H6 Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR271L
• Tested applications: ICC, IP, WB
• Reactivity: Human
• Immunogen: Residues 25-262 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: B7-H6
• UniProt ID: Q68D85
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: B7 homolog 6; putative Ig-like domain-containing p
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human B7-H6 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, K-562, Hep-G2, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784568) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human B7-H6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784568) used for IP at 6 µl/mg lysate.

Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of HEK293T cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784568). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of Hep-G2 cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784568). Secondary: HRP-conjugated goat anti-rabbit IgG.