Rabbit ADE2 Antibody

SKU: orb1522799

Description

ADE2 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 300 and 350
• Target: ADE2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADE2; ADE2H1; AIR carboxylase; AIRC; multifunctional protein ADE2; multifunctional protein ADE2H1; PAIS; phosphoribosylaminoimidazole carboxylase; phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase; phosphoribosylaminoimidazolesuccinocarboxamide synthase; SAICAR synthetase

Detection of human and mouse ADE2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADE2 antibody orb1522799 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse ADE2 by immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Antibody: Affinity purified rabbit anti-ADE2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human ADE2 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ADE2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Quick Database Links

Gene Symbol

ADE2

UniProt

P22234

RefSeq (Protein):NP_006443.1