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RAB20 Rabbit Polyclonal Antibody

SKU: orb101684

Description

RAB20 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Golgi complex, Ras Family

Images & Validation

Tested ApplicationsELISA, IF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
ReactivityRat
Predicted ReactivityBovine, Canine, Equine, Gallus, Human, Mouse, Porcine

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Rab20 (131-234/234aa)
TargetRAB20
Molecular Weight26 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

FLJ20429; RAB20; RAB20 member RAS oncogene family; RAB20_HUMAN; Ras related protein Rab20; Ras-related protein Rab-20.

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RAB20 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RAB20) Polyclonal Antibody, Unconjugated (orb101684) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

RAB20 Rabbit Polyclonal Antibody (orb101684)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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