RAB11FIP3 Antibody

SKU: orb345581

Description

Rab11 FIB3 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:200 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Although the predicted molecular weight of FIP3 is 82.4 kDa, a band corresponding to FIP3 was observed at 120 kDa in human cell lysates and at 150 kDa in mouse cell lysates. The identity of a 40 kDa band, observed in western blotting against both HeLa and 293T cell lysates, is unknown (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD).

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids spanning the carboxyl terminal one-third of human FIP3.
• Target: RAB11FIP3
• Purity: This affinity purified antibody is directed against human FIP3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Rab11 protein from mouse based on approximately 92% protein:protein homology over the region of the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.19 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Rab11-FIP3 antibody, Rabbit anti-Rab11 family-interacting protein 3 antibody, rabbit anti-FIP3-Rab11 antibody, Arfophilin-1, Eferin, EF hands-containing Rab-interacting protein, ARFO1, Rab 11, FIP 3

Quick Database Links

Gene Symbol

RAB11FIP3

UniProt

O75154

RefSeq:7662234