QPRT/QAPRTase Rabbit Polyclonal Antibody

SKU: orb317756

Description

QPRT/QAPRTase Rabbit Polyclonal Antibody

Research Area

Alzheimer's Disease, Amino Acid Metabolism, Huntington's Disease, Neurodegenerative Disease, Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human QPRT/QAPRTase (21-120/297aa)
• Target: QPRT
• Molecular Weight: 31 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

NADC_HUMAN; Nicotinate nucleotide pyrophosphorylase(carboxylating); Nicotinate-nucleotide pyrophosphorylase [carboxylating]; QAPRTase; QPRT; QPRTase; Quinolinate phosphoribosyltransferase [decarboxylating]; Quinolinate phosphoribosyltransferase.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Quinolinate phosphoribosyltransferase, QPRT/QAPRTase) Polyclonal Antibody, Unconjugated (orb317756) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Large intestine tissue lysates, Lane 3: Mouse Kidney tissue lysates, Primary: Anti-QPRT/QAPRTase (orb317756) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kDa, Observed band size: 28 kDa.

Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-QPRT'QAPRTase (orb317756) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31kD, Observed band size: 31kD.

Quick Database Links

Gene Symbol

QPRT

UniProt

Q15274

References

Melanie McReynolds et al. Tracing NAD⁺ metabolism uncovers adaptive coordination between host and microbiome during colitis researchsquare.com, (2025)

PubMed:

Jia-Mei Jiang et al. Asprosin mediates diabetes-associated depression via provoking the cascade of pyroptosis-neuroinflammation-kynurenine pathway disorder in the hippocampus Int Immunopharmacol, (2025)

PubMed: 41338151