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QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody

Catalog Number: orb157190

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb157190
CategoryAntibodies
DescriptionQARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human ProRS (651-750/1512aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW171 kDa
TargetQARS1
UniProt IDP47897
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesBifunctional aminoacyl tRNA synthetase; Bifunction
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NoteFor research use only
Expiration Date12 months from date of receipt.
QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (orb157190) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (orb157190) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (orb157190) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-QARS (orb157190) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 171 kD, Observed band size: 171 kD.

QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody

Tissue/Cell: Rat stomach tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated (orb157190) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.