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Catalog Number | orb1268262 |
---|---|
Category | Antibodies |
Description | PYGM Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Predicted Reactivity | Monkey |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This PYGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from the C-terminal region of human PYGM. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 97 kDa |
Target | PYGM |
UniProt ID | P11217 |
NCBI | P11217 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Glycogen phosphorylase, muscle form, Myophosphoryl Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PYGM using rabbit polyclonal PYGM Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
IHC-P, WB | |
Bovine, Canine, Equine, Human, Porcine, Sheep | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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