You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1258912 |
---|---|
Category | Antibodies |
Description | PYCARD Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein of human ASC / TMS1 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 25kDa |
Target | PYCARD |
UniProt ID | Q9ULZ3 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, A Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ASC/TMS1 antibody (orb1258912) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human placenta using ASC/TMS1 antibody (orb1258912) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using ASC/TMS1 Polyclonal Antibody (orb1258912) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of U937 cells using 3 ug ASC/TMS1 antibody (orb1258912). Western blot was performed from the immunoprecipitate using ASC/TMS1 antibody (orb1258912) at a dilution of 1:1000.
ELISA, ICC, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating