ASC antibody

• Catalog Number: orb338943
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PYCARD
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ASC. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PYCARD
• Entrez: 29108, 66824
• UniProt ID: Q9EPB4, Q9ULZ3
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti ASC antibody, anti CARD5 antibody, anti TMS1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jun Sun 1, Qiuhua Zeng 2, Zhimin Wu 3, Lixin Huang 3, Tao Sun 3, Cong Ling 3, Baoyu Zhang 3, Chuan Chen 4, Hui Wang Berberine inhibits NLRP3 inflammasome activation and proinflammatory macrophage M1 polarization to accelerate peripheral nerve regeneration Neurotherapeutics, 2, e00347 (2024)

PubMed: 38570276

Applications

WB

Reactivity

Mouse

Western blot analysis of ASC expression in HEK293T (A), Raw264.7 (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 24 kD)

Immunohistochemical analysis of ASC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ASC staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).