Pycard antibody

• Catalog Number: orb107673
• Category: Antibodies
• Description: Goat polyclonal to Pycard
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, IHC-P: 10) μg/ml
• Conjugation: Unconjugated
• MW: 21.5
• Target: Pycard (rodent)
• Entrez: 29108
• Protein Sequence: DLTDKLVSYYLES
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Pycard antibody
• Note: For research use only
• Application notes: Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3, KNRK and HeLa cells. Recommended concentration: 10µg/ml.
• Expiration Date: 12 months from date of receipt.

orb107673 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). egative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb107673 Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Egative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb107673 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). egative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western blot analysis of Mouse (A) and Rat (B) Spleen lysates (35ug protein in RIPA buffer) using Pycard antibody