PU.1 antibody

• Catalog Number: orb766159
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PU.1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human SPI1. AA range:181-230
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 32
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SPI1 antibody, anti-Transcription factor PU.1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using PU.1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from COS7 cells, using SPI1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HUVECcells using SPI1 antibody.