PTPN6 Rabbit Polyclonal Antibody

• Catalog Number: orb11369
• Category: Antibodies
• Description: PTPN6 Rabbit Polyclonal Antibody
• Target: PTPN6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human PTPN6 (601-695/695aa)
• UniProt ID: P29350
• RRID: AB_10752030
• MW: 68 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SHP1; 70 kda SHP 1L protein; 70 kda SHP1L protein;
• Research Area: Developmental Biology, G Protein-Coupled Receptors
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PTPN6) Polyclonal Antibody, Unconjugated (orb11369) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human A431 cell lysates, Lane 3: Human HepG2 cell lysates, Primary: Anti-PTPN6 (orb11369) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 65 kDa.