PTPN6 Antibody

• Catalog Number: orb1264586
• Category: Antibodies
• Description: PTPN6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PTPN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human PTPN6.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: PTPN6
• UniProt ID: P29350
• NCBI: P29350
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Tyrosine-protein phosphatase non-receptor type 6,
• Note: For research use only
• Application notes: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of U251 cells, using PTPN6 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of PTPN6 Antibody in HL-60 cell line lysates (35 ug/lane)

Western blot analysis of PTPN6 using rabbit polyclonal PTPN6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2). (2ug/ml)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.