PTPN13 Rabbit Polyclonal Antibody (BF647)

• Catalog Number: orb1598425
• Category: Antibodies
• Description: PTPN13 Rabbit Polyclonal Antibody (BF647)
• Target: PTPN13
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF647
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human FAP1/PTPN13 (801-900/2485aa)
• Tested applications: FC
• Dilution range: Flow-Cyt=3ug/test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: APO1/CD95(Fas) associated phosphatase; Fas associa
• Research Area: Neuroscience, Signal Transduction, Tyrosine Phosph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-FAP1 antibody (orb1598425), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: HL60. Primary Antibody (green line): Rabbit Anti-FAP1 antibody, dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.