PTPLB Rabbit Polyclonal Antibody

SKU: orb317722

Description

PTPLB Rabbit Polyclonal Antibody

Research Area

Cell Biology, Fatty Acid Oxidation

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PTPLB (181-254/254aa)
• Target: HACD2
• Molecular Weight: 28 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

3 hydroxyacyl CoA dehydratase 2; 3-hydroxyacyl-CoA dehydratase 2; HACD2; HACD2_HUMAN; Protein tyrosine phosphatase like(proline instead of catalytic arginine) member b; Protein tyrosine phosphatase like member B; Protein-tyrosine phosphatase-like member B; PTPLB; Very-long-chain(3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 2.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PTPLB) Polyclonal Antibody, Unconjugated (orb317722) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Du145 (Human) Cell Lysate at 30 ug, Primary: Anti-PTPLB (orb317722) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.

Sample: LOVO (Human) Cell Lysate at 30 ug, Primary: Anti-PTPLB (orb317722) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.

Quick Database Links

Gene Symbol

HACD2

UniProt

Q6Y1H2